Cell culture media typically contains a wide range of bioactive molecules with varying concentrations. These are caused by the presence of serum. In contrast, serum-free media is designed for cultivating a specific type of cell or for performing a specific application without interference from these bioactive molecules.

Serum-free media provides numerous advantages over cell culture media that contains serum. These include vastly more consistent performance, easier purification, easier processing, superior control over physiological responsiveness, increased growth, increased productivity. It likewise eliminates the need to sift through media batches, searching for relatively good ones. It also allows the same batch of media to be used as a reliable standard.

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